
Legionella bacteria can cause Legionnaires' disease, a serious type of pneumonia. While most healthy people exposed to Legionella don't get sick, certain people are at a higher risk of infection. Legionella is sometimes found in a building's water supply, and hospitals are not exempt from this. As such, hospitals are required to test for Legionella to varying degrees in different regions. For example, as of July 2016, all general hospitals and residential healthcare facilities in New York State are required to perform an environmental assessment and prepare and implement a sampling and management plan to test their potable water systems for Legionella.
| Characteristics | Values |
|---|---|
| Legionella testing requirements for hospitals | Hospitals are required to perform Legionella testing if they have recorded definite or possible nosocomial Legionella cases within a 6-month period. Hospitals in Texas that perform solid organ and/or bone marrow transplants should have the ability to perform Legionella cultures on-site. All acute-care hospitals in Texas should provide Legionella urinary antigen testing in-house or through a contracted laboratory with results within 48 hours. As of 2016, general hospitals and residential healthcare facilities in New York State are mandated to conduct environmental assessments and implement sampling and management plans for Legionella. |
| Legionella testing procedures | Testing should consider the entire building's water system, with a typical sample size of 250 mL. Testing may be performed onsite or by a qualified technician or commercial laboratory. Testing methods include direct fluorescent antibody testing, immunofluorescence antibody testing, urine antigen testing, and paired serum antibody testing. |
| Legionella control and prevention | Hospitals should develop and implement legionellosis control plans, including educating physicians and staff about Legionnaires' disease, maintaining equipment cleanliness, and implementing water management programs to reduce Legionella growth and spread. |
| Legionella testing results and response | Testing results indicate the presence of Legionella and trigger response activities to control its growth. Response activities are specific to the results, devices, and water systems in the building. |
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What You'll Learn

Hospitals with no identified cases must still test for Legionella
Legionella is a type of bacteria that can cause Legionnaires' disease, a serious form of pneumonia. While most healthy people exposed to Legionella do not get sick, it can be dangerous for certain individuals. As a result, hospitals must be vigilant about testing for and controlling the spread of Legionella, even in the absence of identified cases.
In the United States, the Centers for Disease Control and Prevention (CDC) provides guidance and recommendations for Legionella control and testing in healthcare facilities. The CDC's "Developing a Water Management Program to Reduce Legionella Growth & Spread in Buildings" guide emphasizes the importance of routine Legionella testing, even in buildings without known cases. This routine testing serves as a check on water management programs and provides a baseline to monitor Legionella growth over time.
In addition to CDC recommendations, state and local regulations may also dictate Legionella testing requirements for hospitals. For example, as of 2016, all general hospitals and residential healthcare facilities in New York State are mandated to perform environmental assessments, implement sampling and management plans, and take control measures if Legionella levels exceed acceptable limits. Similarly, Texas's Department of State Health Services (DSHS) provides detailed guidelines for Legionella control and testing in acute-care hospitals, including recommendations for baseline environmental assessments and testing protocols.
Hospitals with no identified cases of Legionella should still adhere to routine testing to ensure the effectiveness of their water management programs. Testing procedures typically involve collecting water samples from various distribution endpoints, such as faucets, showers, and water storage systems. Samples are then analyzed in accredited laboratories, and the results are used to guide response activities aimed at controlling Legionella growth and preventing potential outbreaks.
Furthermore, hospitals should implement preventive measures to minimize the risk of Legionella transmission. This includes educating medical staff about Legionnaires' disease and training them in appropriate diagnostic methods. Hospitals should also maintain high standards of equipment cleanliness, particularly for respiratory care equipment and enteral tubes, by using sterile water for cleaning and flushing. By combining routine testing with preventive strategies, hospitals can effectively safeguard patient health and mitigate the impact of Legionella exposure.
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Legionella testing procedures
Legionella testing is an important procedure for hospitals and healthcare facilities to ensure patient safety and prevent the spread of Legionnaires' disease. Here are detailed instructions for Legionella testing procedures:
Risk Assessment:
- All healthcare facilities should assess their risk of Legionella transmission as part of their legionellosis control plan.
- Environmental, engineering, and patient population factors must be considered to determine the potential for nosocomial transmission.
- Factors such as water chlorination, building design, water distribution systems, and patient populations can influence the risk of Legionella growth and transmission.
Sampling Plan and Collection:
- Develop a comprehensive sampling plan that represents the entire building's water system.
- Consider the key factors for Legionella growth, including temperature, water storage, and plumbing design.
- Collect samples from high-risk areas, such as water distribution endpoints, plumbing outlets, and patient care areas.
- The CDC recommends a 250 mL sample for routine testing, but larger samples or other types, such as swabs or ice, may be necessary for at-risk facilities.
Laboratory Testing:
- Laboratories can use various methods for Legionella testing, including culture, urinary antigen testing (UAT), direct fluorescent antibody testing, molecular methods, and immunological methods.
- Culture and molecular tests are preferred for detecting a range of Legionella species and serogroups, as UAT typically detects only L. pneumophila serogroup 1.
- Ensure that the laboratory is accredited and follows standard operating procedures for Legionella testing.
- Consider factors such as accreditation, expertise, and the ability to share samples during an outbreak investigation when selecting a laboratory.
Interpreting Results:
- Legionella test results are typically reported in colony-forming units (CFU) per volume, with a limit of detection of approximately 10 CFU/mL.
- Any presence of Legionella in the samples should trigger response activities, such as remediation and follow-up testing.
- Consult with the testing laboratory or manufacturer for appropriate interpretation of results, especially if expressed in units other than CFU/mL.
Remediation and Follow-up:
- If Legionella is detected or if there are positive cases of legionellosis, review the sampling and testing procedures for potential errors.
- Confirm the proper functioning of equipment and verify the implementation of the Water Management Plan (WMP) or legionellosis control plan.
- Re-test after remediation to confirm the effectiveness of the response. Address the underlying conditions supporting Legionella growth to prevent recolonization.
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Legionella testing results and response activities
Testing Procedures
Legionella testing can be performed through various methods, including routine and non-routine purposes. Routine testing typically involves assessing the entire building's water system, with a 250 mL sample considered sufficient. The CDC provides guidance on routine testing, recommending an environmental assessment to identify areas with increased risk of Legionella growth and spread.
Non-routine testing may be necessary during outbreaks or as part of public health investigations. Environmental sampling is crucial during outbreaks to inform remediation efforts, and follow-up testing validates the effectiveness of response activities.
Interpreting Results
The CDC has developed a multifactorial approach to interpreting Legionella test results as performance indicators. Results are typically reported in colony-forming units (CFU) per volume, with a limit of detection of approximately 10 CFU/mL. It is important to note that there is no known safe level or type of Legionella, and the presence of any Legionella should trigger response activities.
Response Activities
The detection of Legionella requires a comprehensive response to mitigate the risk to patients and staff. Response activities may include:
- Reviewing sampling and testing procedures to identify potential errors.
- Examining equipment to ensure proper functioning.
- Reviewing records to confirm the implementation of water management plans.
- Re-testing after remediation to confirm the effectiveness of the response.
- Implementing immediate control measures in facilities with populations at increased risk.
Prevention Strategies
In addition to response activities, healthcare facilities should focus on prevention strategies to minimize the risk of Legionella transmission. This includes assessing the risk of legionellosis transmission by considering environmental, engineering, and patient population factors. Baseline water distribution system cultures should be performed if the facility is deemed to have a significant risk of legionellosis transmission.
Furthermore, specific prevention strategies should be developed and implemented by all acute-care hospitals, including education initiatives for physicians and staff, as well as equipment cleanliness protocols.
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Legionella prevention strategies
Legionella is a bacteria found naturally in freshwater environments, but it can become a health concern in human-made building water systems. Legionella causes Legionnaires' disease, which can be fatal. Legionella growth and spread can be controlled by implementing a water management program based on industry standards. Here are some Legionella prevention strategies:
Implement a Water Management Program
Buildings and devices at increased risk for Legionella growth should have a water management program in place. This includes maintaining appropriate water temperatures and disinfectant levels, as well as routine cleaning and disinfection of water systems.
Routine Testing and Sampling
Routine testing for Legionella can help identify the presence of the bacteria in building water systems. Sampling plans should represent the entire building water system, and samples should be collected following standard procedures to ensure accurate results.
Preventative Maintenance and Inspections
Effective maintenance and regular visual inspections of water systems can help prevent Legionella growth. This includes preventing scale buildup, sediment, and gradual water organism accumulation (biofouling). Activity records should be maintained to ensure proper maintenance, including operating procedures, system maintenance dates, and work results.
Biocide Treatment
Periodic biocide treatment in water systems can help prevent or reduce Legionella growth. Halogen oxidizers, ozone, peroxides, and non-oxidizing biocides have been shown to be effective in controlling Legionella when properly used. However, clean water is critical to the effectiveness of biocide treatment.
Education and Training
Physicians, patient-care staff, infection control personnel, and engineers should be educated about Legionella and trained in measures to control nosocomial legionellosis. This includes heightened suspicion for cases of Legionnaires' disease and the use of appropriate diagnostic methods.
These strategies aim to prevent Legionella growth and reduce the risk of Legionnaires' disease in healthcare facilities and other buildings with complex water systems. Each facility should assess its specific risks and implement tailored prevention and control measures.
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Legionella testing requirements for cooling towers
Legionella is a type of bacteria that can grow in water systems and cause severe respiratory diseases, including Legionnaires' disease and Pontiac fever. It is essential to test for Legionella in cooling towers regularly as they are one of the primary sources of Legionella outbreaks. Cooling towers are used in many commercial and industrial buildings to cool water used in heating, ventilation, and air conditioning (HVAC) systems. The water in these towers is warm and exposed to air, providing ideal conditions for Legionella bacteria to grow and multiply.
The testing frequency for Legionella in cooling towers depends on various factors, including local and state regulations, previous Legionella issues, and the unique risk factors of the water system. The general recommendation is to test cooling tower water at least quarterly. However, if there have been previous issues with Legionella, more frequent testing, such as weekly or monthly, may be necessary until the system is functioning well with the current prevention strategy. It is important to note that there is no penalty for testing more often than the minimum required, as the priority is to ensure the safety of people and the optimal performance of the tower.
When conducting Legionella testing in cooling towers, it is crucial to hire a qualified individual or work with a Legionella expert to determine the most effective testing frequency and locations. The cooling tower basin, where water is stored and most exposed to air, should be tested at least twice a year. The drift eliminators, fill material, water distribution system, and surrounding air are also key areas for Legionella testing. A Legionella risk assessment is essential to identify areas with increased risk and develop a suitable water treatment program for the cooling tower.
In the context of healthcare facilities, such as hospitals, the prevention and control of Legionella are crucial. Hospitals should implement legionellosis control plans and assess their risk of legionellosis transmission. Environmental, engineering, and patient population factors should be considered to determine the potential for nosocomial transmission. Baseline water distribution system cultures and environmental assessments should be performed if the facility is at significant risk. Hospitals should also provide Legionella urinary antigen testing in-house or collaborate with laboratories that can report test results within a specified timeframe. Additionally, hospitals that care for patients with solid organ or bone marrow transplants should have the capability to perform Legionella cultures on-site.
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Frequently asked questions
Hospitals are not always required to test for Legionella. However, in certain regions, such as New York State, hospitals are mandated to perform an environmental assessment and implement a sampling and management plan for Legionella in their potable water systems. Hospitals should also assess their risk of Legionella transmission and conduct routine testing if there is a significant risk.
Legionella is a type of bacteria that can cause Legionnaires' disease, a serious form of pneumonia. Certain individuals, such as those with chronic health conditions or weakened immune systems, are at an increased risk of developing Legionnaires' disease if exposed to Legionella. Therefore, hospitals should implement prevention strategies and control measures to protect their patients and staff.
Hospitals should develop and implement a water management plan (WMP) to reduce Legionella growth and spread. This includes identifying potential sources of exposure, such as water aerosols from faucets, showers, and medical equipment, and establishing control measures and intervention strategies. Additionally, routine Legionella testing can help hospitals understand the quality of their water and effectiveness of their WMP.




















