
Semen analysis, also known as a sperm count test, is a test that assesses a man's fertility or confirms the success of a vasectomy. It is one of the first tests done to evaluate a man's fertility and can be carried out in a hospital or fertility clinic. The test measures the health and viability of a man's sperm, including sperm count, sperm concentration, sperm movement (motility), and sperm shape (morphology). To perform the test, a semen sample is collected through masturbation in a private room at a hospital or clinic, or at home. The sample must be kept at body temperature and delivered to the testing facility within 30 to 60 minutes of leaving the body.
| Characteristics | Values |
|---|---|
| Purpose | To assess fertility or confirm the success of a vasectomy |
| Semen sample collection | Masturbation in a private room at a fertility clinic or lab, or at home |
| Sexual activity before test | Abstain from sexual activity for 2-7 days before the test |
| Time since vasectomy | Test usually takes place 8-12 weeks after the procedure |
| Frequency of tests | Two or three separate tests conducted at least seven days apart over two to three months |
| Volume | 1.5 to 7.6 milliliters |
| Sperm count | More than 39 million sperm in the entire sample |
| Sperm concentration | 15 to 259 million sperm per milliliter of semen |
| Sperm movement (motility) | Over 40% of sperm should be moving efficiently |
| Sperm shape (morphology) | Over 4% of sperm should have a normal shape |
| pH | 7.2 to 8.0 pH |
| Time to liquefaction | 15 to 30 minutes after collection |
| White blood cells (leukocytes) | Less than 1 million white blood cells per 1 milliliter of semen |
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What You'll Learn

Semen analysis
Procedure
It is recommended that individuals abstain from sexual activity, including intercourse and masturbation, for two to seven days before the sample is collected. This ensures that the sperm count is at its highest level for the most accurate analysis.
Analysis
The semen sample is then examined in a lab using a microscope. The following characteristics are evaluated:
- Volume: The amount of semen in the sample, ideally between 1.5 to 7.6 milliliters.
- Sperm count: The number of sperm in the entire sample, ideally more than 39 million sperm.
- Sperm concentration: The number of sperm per milliliter of semen, ideally between 15 to 259 million sperm per milliliter.
- Sperm movement (motility): The percentage of sperm that are moving efficiently, ideally over 40%.
- Sperm shape (morphology): The percentage of sperm with a normal shape, ideally over 4%.
- Time to liquefaction: How quickly the semen changes into a fluid, ideally within 15 to 30 minutes after collection.
- PH: A normal semen sample should have a pH between 7.2 and 8.0.
- White blood cell count: A high number of white blood cells may indicate an infection affecting fertility.
Results
The results of the semen analysis will be discussed with a doctor. If the semen analysis was performed to confirm the success of a vasectomy, the desired result is no sperm or a few non-moving sperm in the semen. Abnormal results may indicate the need for additional tests to identify any underlying conditions affecting fertility.
Risks and Considerations
There are no known risks associated with semen analysis. However, it is important to note that lubricants and condoms containing lubricants can affect the results. Additionally, substances like alcohol, tobacco, caffeine, recreational drugs, prescription medications, and herbal medicines can also impact the accuracy of the test. Therefore, individuals should inform their healthcare provider about any substances or medications they are consuming before the test.
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Sperm count test procedure
A sperm count test, also known as a semen analysis, is a test that assesses male fertility or confirms the success of a vasectomy. The test evaluates the sperm count, sperm activity (motility), sperm shape (morphology), volume, liquefaction, pH, and the presence of white blood cells. Semen is a whitish-gray fluid released from the penis during ejaculation, containing sperm, sugar, and protein substances.
To prepare for a sperm count test, it is recommended to refrain from sexual activity, including masturbation, for two to seven days prior to the test. This ensures that the sperm count is at its highest level for the most accurate analysis.
The test can be performed at a fertility clinic or lab, where a semen sample is collected. The preferred method of collection is through masturbation, which can be done at home or in a private room at the clinic. It is important to maintain clean hands and genitalia prior to collection. The sample should be kept at room temperature and delivered to the lab within 30 to 60 minutes.
Multiple semen samples may be required, spaced a few weeks apart, as sperm counts can vary daily. Doctors often conduct two or three separate tests to comprehensively assess sperm health. The test results are then analyzed to determine fertility or identify any abnormalities.
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Preparing for a sperm count test
A sperm count test, also known as a semen analysis, is a test that assesses your fertility or confirms the success of a vasectomy. It involves providing a semen sample, which will be evaluated for sperm shape, movement, pH, volume, liquefaction, and appearance. Here are some things to keep in mind when preparing for a sperm count test:
Abstain from sexual activity:
It is recommended to refrain from any sexual activity, including masturbation, that causes ejaculation for two to four days before the test. This ensures that your sperm count is at its highest level and provides the most accurate analysis.
Avoid certain substances:
Refrain from consuming alcohol, caffeine, and recreational drugs such as cocaine and marijuana for at least two to five days before the test. Additionally, discontinue the use of any herbal medications, such as St. John's wort and high doses of echinacea, as instructed by your healthcare provider. Avoid hormone medications unless advised otherwise by your doctor.
Maintain hand and penis hygiene:
Ensure that your hands and penis are clean before providing the semen sample. It is recommended to take a shower and wash your hands and genital area with soap and water.
Choose a collection method:
You can choose to provide the semen sample at home or in a private room at the fertility clinic or lab. If you choose to collect the sample at home, keep it at body temperature by placing it in your coat's inside pocket. Get the sample to the lab within 30 minutes to one hour of ejaculation, as sperm is delicate and has a limited lifespan outside the body.
Bring a valid photo ID:
Don't forget to bring a government-issued photo ID, such as a driver's license or passport, to the fertility clinic or lab to verify your identity.
Remember to follow any specific instructions provided by your healthcare provider, as they may have additional recommendations or requirements for preparing for the sperm count test.
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Interpreting sperm count test results
Semen analysis, also known as a sperm count test, is a test that assesses fertility or confirms the success of a vasectomy. The test measures the amount and quality of a man's semen and sperm. Semen is the fluid that contains sperm, and it is released during ejaculation. The test evaluates the volume of semen, sperm count, sperm motility (movement), and sperm morphology (shape and size).
Sperm count is typically measured in millions per millilitre of semen. A total motile count of over 20 million is considered normal, and a count of less than 20 million may decrease the chances of a successful pregnancy. However, even with a higher count, the chances of pregnancy may not increase. A count of fewer than 5 million is considered severely low. Sperm motility is also crucial, with over 50% providing a better chance of pregnancy. At least 60% of sperm should have a normal shape and exhibit forward movement.
The appearance of semen is also evaluated. It should typically be whitish to grey and opalescent. Variations in colour may indicate issues such as the presence of blood or jaundice. The semen's pH, liquefaction, and volume are also assessed. The volume should be greater than 2 millilitres, as a low volume could indicate a reduced amount of sperm available for fertilisation.
It is important to note that sperm counts can vary daily, and multiple tests are often conducted to get a comprehensive understanding of sperm health. Additionally, the interpretation of results may vary between laboratories, and other factors such as age, health history, and substances used can influence the results. Therefore, it is essential to consult a healthcare provider to understand the specific implications of your test results.
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At-home vs in-lab testing
A semen analysis is a test that assesses fertility (the ability to conceive) or confirms the success of a vasectomy. It can be done at home or in a laboratory.
At-home testing
At-home testing kits are cheaper and easier than seeing a doctor. They can be a valuable tool for family planning and can provide some encouragement that you're fertile. For example, if you have certain risk factors for infertility, like a history of chemotherapy, a home test could indicate whether you're fertile.
However, home tests cannot provide a full picture of your fertility. They differ from the kind of testing done in an andrology laboratory, which gives a much more thorough assessment. For instance, they cannot determine the exact sperm concentration, the percentage of healthy sperm, the volume of ejaculate, or the acidity (pH) of semen.
In-lab testing
In an andrology lab, your semen will be evaluated under a microscope and by a computer, and also looked at by a healthcare provider. Lab testing can determine your exact sperm concentration, the percentage of healthy sperm, the volume of ejaculate, the acidity (pH) of your semen, and the size and shape of your sperm.
Home tests can be a good starting point, but for a proper, accurate male fertility test, you should be evaluated by a physician and have a semen sample analyzed in a professional andrology laboratory.
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Frequently asked questions
Doctors recommend that you refrain from sexual activity that results in ejaculation for 2 to 7 days before the test. This ensures that your sperm count is at its highest level. You should also avoid alcohol, caffeine, and drugs such as cocaine and marijuana two to five days before the test.
The preferred method of producing a semen sample is through masturbation. This can be done at home or in a private room at a fertility clinic or lab. The latter may provide visual masturbatory material, but you can also use your smartphone. It is important to keep your hands and penis clean before you masturbate.
The semen sample must be kept at body temperature and delivered to the testing facility within 30 to 60 minutes of leaving the body. The sample will then be analysed for sperm count, sperm concentration, sperm movement (motility), sperm shape (morphology), and time to liquefaction.









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